2型糖尿病与糖尿病肾病患者微炎症及肠道微生物多样性分析

目的 分析2型糖尿病(T2DM)及糖尿病肾病(DKD)患者微炎症情况和肠道微生物多样性。 方法 将2016年4月至2019年7月在我院进行治疗的68例T2DM患者(T2DM组)和57例DKD患者(DKD组)纳入研究,选择同期于我院进行健康体检的36例志愿者作为对照组。收集3组对象一般资料、血液标本和粪便标本,测定血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDLC)、糖化血红蛋白(HbAlc)、空腹血糖(FBG)、超敏C反应蛋白(hsCRP)、白细胞介素6(IL6)水平,并对肠道细菌进行16S rDNA序列测序。比较3组对象一般资料,血液指标,肠道菌群门水平构成情况,肠道菌群多样性,肠道菌属差异性,并对患者炎性指标与菌群种类进行相关性分析。 结果 DKD组患者糖尿病病程长于T2DM组(P结论 2型糖尿病患者普遍存在微炎症和肠道菌群失衡,微炎症程度与肾脏病变和肠道菌群数量密切相关。T2DM与DKD患者在肠道菌群结构上具有一致性和差异性,肠道菌群检测有可能成为预测T2DM患者发生肾脏病变的风险指标。

Analysis of microinflammation and intestinal microbial diversity in patients with type 2 diabetes mellitus and diabetes kidney disease

Objective To observe microinflammation and intestinal microbial diversity in patients with type 2 diabetes mellitus (T2DM) and diabetes kidney disease (DKD). Methods A total of 68 patients with T2DM (T2DM group), 57 patients with DKD (DKD group) and 36 healthy individuals (control group) in our hospital from April 2016 to July 2019 were enrolled. General information and specimens of blood and stool were collected. The levels of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC), high density lipoprotein cholesterol (HDLC), glycated hemoglobin (HbAlc), fasting blood glucose (FBG), highsensitivity Creactive protein (hsCRP) and interleukin6 (IL6) were detected. 16S rDNA gene sequence of intestinal bacteria was sequenced. General information, blood indexes, composition of intestinal phylum, diversity of intestinal flora and difference of intestinal genus were compared among the three groups. Then the correlation between the inflammatory indexes and the types of bacteria was analyzed. Results The course of diabetes in DKD group was longer than that in T2DM group (PConclusion Microinflammation and intestinal flora imbalance are common in patients with type 2 diabetes, and the degree of microinflammation is closely related to renal disease and intestinal flora concentration. The structure of intestinal flora in patients with T2DM and DKD is consistent and different, so the detection of intestinal flora may be a risk indicator for predicting renal diseases in patients with T2DM.