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Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi-automatic quantitative analysis
Authors:Carmen Quintana  Carmen Lpez-Iglesias  Noel Bonnet  Stephane Lebonvallet
Institution:Carmen Quintana,Carmen López-Iglesias,Noel Bonnet,Stephane Lebonvallet
Abstract:Summary— In a previous paper (Quintana et al, Biol Cell 72, (1991) 167–180) we reported the anti-ovalbumin antibody immunolabeling in the cytoplasm of tubular gland cells from cryofixed, cryosubstituted and acrylic-resin embedded quail oviduct. To confirm these preliminary visual observations, we have carried out a semi-automatic quantitative study of immunogold labeling. The quantitative results confirm the previous data. In addition, we found a significantly higher immunolabeling with low temperature Lowicryl K11M embedding procedure as compared with high temperature LR White embedding.
Keywords:quantitative immunolabeling  cryomethods  quail  oviduct  ovalbumin
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