| 32株金黄色葡萄球菌分子分型及肠毒素分析 |
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| 引用本文： | 于淼, 张眉眉, 耿英芝, 等. 32株金黄色葡萄球菌分子分型及肠毒素分析[J]. 中国微生态学杂志, 2021, 33(6): 658-661. doi: 10.13381/j.cnki.cjm.202106007 |
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| 作者姓名： | 于淼 张眉眉 耿英芝 魏彤竹 张铭琰 范文今 |
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| 作者单位： | 辽宁省疾病预防控制中心,辽宁 沈阳 110005 |
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| 基金项目： | 辽宁省自然科学基金指导计划(20180550982) |
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| 摘 要： | 目的 对辽宁省内2016-2018年分离出的食源性金黄色葡萄球菌采用脉冲场电泳(PFGE)和肠毒素分型进行分析,为今后公共卫生等领域提供技术保障.方法 将32株金黄色葡萄球菌用限制性内切酶SmaI酶切以进行PFGE分析,并用BioNumerics(7.6版本)软件对分离株的指纹图谱进行聚类分析;用PCR方法对菌株进行肠...
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| 关 键 词： | 食源性金黄色葡萄球菌 分子分型 脉冲场凝胶电泳 PCR |
Molecular typing and enterotoxin analysis of 32 strains of Staphylococcus aureus |
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| YU Miao, ZHANG Meimei, GENG Yingzhi, et al. Molecular typing and enterotoxin analysis of 32 strains of Staphylococcus aureus[J]. Chinese Journal of Microecology, 2021, 33(6): 658-661. doi: 10.13381/j.cnki.cjm.202106007 |
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| Authors: | YU Miao ZHANG Meimei GENG Yingzhi WEI Tongzhu ZHANG Mingyan FAN Wenjin |
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| Affiliation: | Liaoning Center for Disease Prevention and Control, Shenyang, Liaoning 110005, China |
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| Abstract: | Objective To analyze foodborne Staphylococcus aureus isolated in Liaoning from 2016 to 2018 by using pulsed field electrophoresis (PFGE) and enterotoxin typing to provide technical support for public health. Methods A total of 32 strains of Staphylococcus aureus were digested with restriction enzyme SmaI for PFGE analysis, their fingerprints were analyzed with BioNumerics (version 7.6), and the enterotoxins were detected using PCR. Results The similarity coefficients of PFGE patterns of the 32 foodborne Staphylococcus aureus were between 26.8% and 100.0%, and 31 PFGE patterns were obtained through cluster analysis. Each strain carries 1 to 3 virulence genes, of which SEH gene was most frequently carried each year. Conclusion Staphylococcus aureus can be typed using PFGE and PCR, both of them have good typing ability, high identification and good traceability to the strains. |
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| Keywords: | Foodborne Staphylococcus aureus Molecular typing Pulse field gel electrophoresis PCR |
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