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Arg3]substance P and neurokinin A from chicken small intestine
Authors:J M Conlon  S Katsoulis  W E Schmidt  L Thim
Affiliation:1. Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft, Göttingen F.R.G.;2. Department of Medicine, University of Göttingen, Göttingen F.R.G.;3. Novo Research Institute, Bagsvaerd Denmark;1. Department of Anthropology, University of Washington, Seattle, WA, United States;2. Center for Studies in Demography and Ecology, University of Washington, Seattle, WA, United States;3. USC-Office of Population Studies Foundation, Inc, University of San Carlos, Cebu, Philippines;4. Department of Anthropology, Sociology, and History, University of San Carlos, Cebu, Philippines;1. Korea Institute of Machinery and Materials, 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 34103, Republic of Korea;2. Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea;1. Department of Neurology, Daping Hospital, Army Medical University, 10 Changjiang Branch Road, Yu-Zhong District, 400042 Chongqing, China;2. Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, 10 Changjiang Branch Road, Yu-Zhong District, 400042 Chongqing, China;1. Department of Psychiatry, Harvard Medical School, Boston, MA 02115, USA;2. Laboratory for Psychiatric and Molecular Neuroscience, McLean Hospital, Belmont, USA;3. Laboratory for Genetic Neuropharmacology, McLean Hospital, Belmont, USA;4. Psychology Research Laboratory, McLean Hospital, Belmont, USA;5. Translational Psychiatry Laboratory, McLean Hospital, Belmont, MA 02478, USA;1. College of Medicine, Nankai University, Tianjin, China;2. Medical School of Chinese PLA, Beijing, China;3. Department of Neurology, the First Medical Center, Chinese PLA General Hospital, Beijing, China;4. Department of Neurology, PLA Rocket Force Characteristic Medical Center, Beijing, China
Abstract:Using antisera specific for NH2-terminal and COOH-terminal regions of substance P and for the COOH-terminal region of neurokinin A, peptides with tachykinin-like immunoreactivity were isolated from extracts of chicken small intestine. The peptide Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 differs from human substance P by substitution of the lysyl residue by an arginyl residue at position 3. Synthetic [Arg3]substance P showed identical chromatographic and immunochemical properties to chicken substance P and was equipotent with substance P in contracting the guinea pig ileum. A second peptide His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 isolated from the extracts is identical to human neurokinin A. A third peptide was immunoreactive towards the COOH-terminally directed anti-serum to substance P only but was not characterized structurally in this study.
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