Developmental changes in the fine structure of the salivary gland of Trichosia pubescens (Morgante) (Diptera : Sciaridae) in relation to puffing activity |
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| Institution: | 1. St-Patrick Research Group in Basic Oncology, Centre Hospitalier Universitaire de Québec-Université Laval Research Centre (Hôtel-Dieu de Québec), Quebec City, Québec, Canada G1R 2J6;2. Laval University Cancer Research Centre, Quebec City, Québec, Canada G1R 2J6;2. Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China;3. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, PR China;4. School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China;5. South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), PR China;1. State Key Laboratory of Biocontrol, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China;2. Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;3. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/ Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;4. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, PR China;1. MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;2. Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China;3. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou, PR China;4. School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China;1. State Key Laboratory for Biocontrol / School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China;2. Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, PR China;1. Department of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway;2. Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway;3. Department of Gynecology, Oslo University Hospital and Institute of Clinical Medicine, University of Oslo, Oslo, Norway |
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| Abstract: | Two differentiated sections (S1 and S2) of the salivary gland of Trichosia pubescens (Morgante) (Diptera : Sciaridae) have been examined by electron microscopy for fine structural alterations that occur in the cell cytoplasm during larval development. Such changes have been correlated with the puffing patterns of the polytene chromsomes. During stage 1 (end of the 3rd instar to mid 4th instar), the puffing pattern and the ultrastructure of S1 and S2 cells are rather constant. Nevertheless, marked differences are noted when the puffs and the fine structure of the 2 sections are compared. In S1, secretory material is concentrated and eliminated as membrane-bound granules, while in S2, secretory granules are not detected and the elimination of secretion seems to occur continuously. At stage 2 (end of the 4th instar), the puffing pattern undergoes considerable alterations simultaneously with the appearance of many ultrastructural modifications. In S1, the morphological aspect of the secretory granules is altered, while in S2 a decline in the secretory activity is detected. At stage 3 (older 4th-instar larvae), most of DNA puffs are active, there being no striking differences in the puffing pattern between S1 and S2. This stage is marked by the onset of gland histolysis, with the appearance of an intense autophagic activity of lysosomes in S1 and S2. As histolysis progresses during stage 4 (prepupae and early pupae) the activity of the polytene chromosomes decreases; most of the cells present a large number of autophagic vacuoles and an increasing disorganization of the cytoplasm, leading to the final lysis of the gland. |
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