Analysis of Dip2B Expression in Adult Mouse Tissues Using the LacZ Reporter Gene |
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| Authors: | Rajiv Kumar Sah Noor Bahadar Fatoumata Binta Bah Salah Adlat Zin Mar Oo Luqing Zhang Fawad Ali M S Zobaer Xuechao Feng Yaowu Zheng |
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| Affiliation: | 1.Key Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China; (R.K.S.); (N.B.); (F.B.B.); (S.A.); (Z.M.O.); (L.Z.);2.WISH Biotechnologies, Beihu Scinece Park B, Changchun 130000, China;3.Department of Chemistry, Bacha Khan University, Charsadda 6431, KP, Pakistan;4.McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX 77030, USA; |
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| Abstract: | Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip2B in other somatic tissues remain unknown. To better visualize Dip2B expression and to provide insight into the roles of Dip2B during postnatal development, we used a Dip2btm1a(wtsi)komp knock-in mouse model, in which a LacZ-Neo fusion protein is expressed under Dip2b promoter and allowed Dip2B expression to be analyzed by X-gal staining. qPCR analyses showed that Dip2b mRNA was expressed in a variety of somatic tissues, including lung and kidney, in addition to brain. LacZ staining indicated that Dip2B is broadly expressed in neuronal, reproductive, and vascular tissues as well as in the kidneys, heart, liver, and lungs. Moreover, neurons and epithelial cells showed rich staining. The broad and intense patterns of Dip2B expression in adult mice provide evidence of the distribution of Dip2B in multiple locations and, thereby, its implication in numerous physiological roles. |
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| Keywords: | Dip2b LacZ nervous system reproductive system vascular system respiratory system |
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