In vitro release of lipophorin from the fat body of nondiapause and diapause larvae of the Southwestern corn borer,Diatraea grandiosella |
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| Institution: | 1. Temple University, School of Sport, Tourism and Hospitality Management, 309 Speakman Hall, 1810?N. 13th St., Philadelphia, PA 19122, USA;2. Institute of Sport, Exercise and Active Living, Victoria University, PO Box 14428, Melbourne, VIC, 8001, Australia;3. Faculty Business and Law, Deakin University, 221 Burwood Highway, Burwood VIC 3125, Australia;4. Think Company, 1315 Walnut Street, Suite 201, Philadelphia, PA 19107, USA;1. Department of Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China;2. College of Mathematics and Physics, Shanghai University of Electric Power, Shanghai 201306, China;3. School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore;4. Shanghai Key Laboratory of High-resolution Electron Microscopy, ShanghaiTech University, Shanghai 201210, China;1. Department of Dermatology and Venereology, Hamad Medical Corporation, Doha, Qatar;2. HMC Translational Research Institute, Hamad Medical Corporation, Doha, Qatar;3. Weill Cornell Medicine-Qatar, Doha, Qatar;4. College of Medicine, Qatar University, Medical School, Doha, Qatar;5. Department of Experimental Pain Research, CBTM Mannheim, Heidelberg University, Mannheim, Germany;6. Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary;7. Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;8. Department of Neuropathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;1. Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA, USA;2. Witten/Herdecke University, Witten, Germany;1. Centre for Clean Energy and Nano Convergence (CENCON), Hindustan Institute of Technology and Science (HITS), Padur, Kelambakkam, Chennai 603103, India;2. Quantum Functional Semiconductor Research Centre (QSRC), Nano information Technology Academy(NITA), Dongguk University, 30 Pildong-ro 1-gil, Jung-gu, Seoul 04620, South Korea |
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| Abstract: | The release of lipophorin and total protein was examined from the fat body of nondiapause and diapause larvae of the southwestern corn borer, Diatraea grandiosella, incubated in vitro in Grace's medium. The characteristics of the released lipophorin were compared to those of the high-density lipophorin present in the hemolymph of nondiapause and diapause larvae. Over a 4 h incubation period, the fat body of nondiapause larvae released about 1.5 times more total protein and 2 times more lipophorin per mg dry weight than did that of diapause larvae. Lipophorin isolated from the medium in which fat bodies of nondiapause and diapause larvae had been incubated and from the plasma of nondiapause and diapause larvae had similar mean densities of 1.115, 1.112, 1.117 and 1.119 g/ml, respectively. Although the lipid classes detected in lipophorin isolated from the fat body incubation medium and hemolymph were identical, more polar lipids and less diacylglycerol were associated with lipophorin isolated from fat body incubation medium then were associated with lipophorin isolated from the hemolymph. Sterols accounted for about 11% of the total lipids of lipophorin isolated from the fat body incubation medium, whereas they accounted for about 20% of the total lipids of lipophorin from hemolymph. We conclude that the fat body of feeding nondiapause larvae and nonfeeding diapause larvae releases high-density lipophorin. |
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