Antisera against AKHs and AKH precursors for experimental studies of an insect neurosecretory system |
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| Institution: | 1. School of Transportation, Wuhan University of Technology, Wuhan 430063, PR China;2. Chongqing Energy College, Chongqing 402260, PR China;3. Postdoctoral Workstation of Chongqing Bureau of Geology and Minerals Exploration, Chongqing 401121, PR China;1. State Key Laboratory of Earthquake Dynamics, Institute of Geology, China Earthquake Administration, P.O. Box 9803, Beijing 100029, China;2. Key Laboratory of Active Tectonics and Volcanos, Institute of Geology, China Earthquake Administration, P.O. Box 9803, Beijing 100029, China;1. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China;2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China;3. Earth Systems Research Center, Institute for the Study of Earth, Oceans, and Space, University of New Hampshire, Durham, NH, 03824, USA;4. Institute of Grassland Science, Northeast Normal University and Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun, 130024, China;1. Research Scholar, Gujarat Technological University, Ahmedabad, Gujarat, India;2. Associate professor, Mechanical Engineering Department, C.K. Pithawala College of Engineering and Technology, Surat, Gujarat, India;1. Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raibareily Road, Lucknow, Uttar Pradesh, India;2. Department of Nephrology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raibareily Road, Lucknow, Uttar Pradesh, India |
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| Abstract: | Three different oligopeptides based on the amino acid sequence of Schistocerca gregaria AKH I and AKH II were designed and synthesized. They were used to raise rabbit antisera capable of differentiating between AKH I and II and of recognizing the identified precursors of the AKHs (P1 and P2). Analogue I (Lys-Tyr-Thr-Pro-Asn-Trp-Gly-Thr-NH2) generated AKH I specific antisera, analogue II (Lys-Tyr-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NH2) generated AKH II specific antisera. A non-amidated analogue of AKH I called analogue III (Lys-Tyr-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-Thr-OH) generated antisera capable of recognizing both the P1 and P2 AKH precursors. The antiserum recognizing the precursor (antiserum IIIa) can be used to monitor experimentally induced changes in precursor levels in the locust corpus cardiacum. The antisera described here are discussed in terms of their utility in studying the insect neurosecretory system. |
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