Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis,Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology |
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| Authors: | Goldin John Nikhil Shri Sahajpal Ashis K Mondal Sudha Ananth Colin Williams Alka Chaubey Amyn M Rojiani Ravindra Kolhe |
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| Institution: | 1.Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA; (G.J.); (N.S.S.); (A.K.M.); (S.A.); (C.W.); (A.C.);2.Bionano Genomics Inc., San Diego, CA 92121, USA;3.Department of Pathology, Penn State University College of Medicine, Hershey, PA 16802, USA; |
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| Abstract: | This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19. |
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| Keywords: | next-generation sequencing COVID-19 challenges diagnostic assay |
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