Trehalose synthase and trehalase behaviour in yeast cells in anhydrobiosis and hydrobiosis |
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| Institution: | 1. Dipartimento di Medicina ed Oneologia Sperimentale, Sezione Biochimica, Università di Torino, Torino, Italia;2. Dipartimento di Morfbfisiologia Veterinaria, Università di Torino, Torino, Italia;1. Chaire Industrielle de Recherche sur la Construction Ecoresponsable en Bois (CIRCERB), Pavillon Gene-H-Kruger, Université Laval, 2425, rue de la terrasse, Québec, Québec, G1V0A6, Canada;2. Department of Civil Engineering, Université de Sherbrooke, 2500, boul. de l’Université, Sherbrooke, QC, J1K 2R1, Canada;3. FPInnovations, 319 rue Franquet, Québec, QC, G1P 4R4, Canada;1. Université d’Orléans, UPRES EA 1207, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), 45067 Orléans Cedex 2, France;2. INRA, USC1328, Arbres et Réponses aux Contraintes Hydriques et Environnementales (ARCHE), 45067 Orléans Cedex 2, France;3. Université de Toulon, EA 3819, Laboratoire Processus de Transferts et d’Echanges dans l’Environnement (PROTEE), 83957 La Garde Cedex, France;4. Université François Rabelais de Tours, EA 2106, Biomolécules et Biotechnologies Végétales (BBV), 37200 Tours, France;1. Department of Studies in Botany, University of Mysore, Manasasgangotri, Mysuru 570 006, Karnataka, India;2. Department of Studies in Biotechnology, University of Mysore, Manasasgangotri, Mysuru 570 006, Karnataka, India;1. State Key Laboratory of Cotton Biology, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, 475001, China;2. College of Life Sciences, Shanghai Normal University, Guilin Road 100, Shanghai, 200234, China |
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| Abstract: | - 1.I. Trehalose synthase and trehalase behaviour has been analysed in cultured yeast cells isolated from baker's yeast to increase the understanding of the mechanisms involved in trehalose content modifications observed in anyhydrobiois and hydrobiosis.
- 2.2. After desiccating yeast cells to a constant weight, trehalose levels sharply increased, whereas the glycogen content decreased, trehalose synthase was stimulated and trehalase was significantly inhibited.
- 3.3. In desiccated cells after a rehydration for 15 min, trehalose levels dropped, the glycogen content further decreased, the activity of trehalose synthase declined while that of trehalase was dramatically stimulated.
- 4.4. After rehydration for 12hr, while the trehalose and glycogen content decreased even more, the behaviour of the two enzymes was completely reversed, trehalose synthase being activated and trehalase inhibited.
- 5.5. The reasons for such impressive enzyme activity alterations in desiccated and rehydrated cells for the moment remain unknown.
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