Identification and molecular analysis of the cuticle protein genes of Dacus oleae |
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Institution: | 1. National Hellenic Research Foundation, 48, Vas. Constantinou Av., Athens 116 35 Greece;2. Department of Biology, Faculty of Sciences, University of Patras, Patras 260 00, Greece |
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Abstract: | A 11.9 Kb DNA segment of the Dacus oleae genome that contains three cuticle protein genes has been cloned and characterized. These three genes are clustered within 8.04 Kb of DNA; the restriction sites for eight enzymes and the organization of the cuticle genes in this 11.9 Kb cloned fragment were determined. Using this clone as a radioactive probe, it was shown that the three cuticle genes are expressed as poly(A) RNA in the epidermis of late third instar larvae but are not abundantly expressed in other tissues. |
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