Ultrastructure of host tissues exposed to the calyx fluid of the parasitoid,Campoletis sonorensis (Cameron) (Hymenoptera: Ichneumonidae) |
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| Institution: | 1. Department of Entomology, Clemson University, Clemson, SC 29634, U.S.A.;2. Department of Entomology, 237 Russell Labs, University of Wisconsin, Madison, WI 53706, U.S.A.;3. Department of Biology, Medawar Building, University College, London, Gower Street, London WC1E 6BT, U.K.;4. Department of Entomology, Texas A&M University, College Station, TX 77843, U.S.A.;1. Faculty of Mathematics and Sciences, Department of Biology and Ecology, University of Niš, Višegradska 33, 18000, Niš, Serbia;2. Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000, Belgrade, Serbia;1. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;2. The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;3. Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;4. Fishberg Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA;5. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB21QR, UK;6. Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province 361102, China;7. Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;8. Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, China;1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China;2. Department of Crop Protection, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;3. Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, Athens, Greece |
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| Abstract: | Campoletis sonorensis (Cameron) (Hymenoptera: Ichneumonidae) is a solitary endoparasitoid of Heliothis virescens. The lateral oviducts of the female parasitoid contain a particulate suspension called calyx fluid. The particles in calyx fluid are a polydnavirus (CsV) which, when injected into last-instar H. virescens, stimulates degeneration of the host's prothoracic glands. In order to determine if CsV-induced degeneration is specific to prothoracic glands, last-instar H. virescens larvae were injected with C. sonorensis calyx fluid. After 4 days, a variety of host tissues were dissected from both calyx fluid-injected and uninjected control larvae and fixed for transmission electron microscopy. Prothoracic glands from injected larvae were ultrastructurally degenerated by 4 days post-injection, whereas control glands remained intact. Other tissues from calyx fluid-injected larvae (tracheal epithelia, corpora allata, Malpighian tubules, fat body, skeletal muscle, and the brain) showed no signs of ultrastructural degeneration or gross abnormalities as compared with control tissues. These observations suggested that CsV-induced degeneration is specific to the host's prothoracic glands. |
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