The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties. |
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| Authors: | L Tora A Mullick D Metzger M Ponglikitmongkol I Park P Chambon |
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| Affiliation: | Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France. |
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| Abstract: | We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400. |
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