Purification and characterization of chitinase from Alcaligenes xylosoxydans |
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Authors: | Vaidya Rajiv Roy Satarupa Macmil Simone Gandhi Sapan Vyas Pranav Chhatpar H S |
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Institution: | (1) Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S, University of Baroda, Vadodara-390 002 Gujarat, India |
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Abstract: | Extracellular chitinase from Alcaligenes xylosoxydans was purified to electrophoretic homogeneity using affinity and gel filtration chromatography. The molecularmass of chitinase was estimated to be 45 kDa and44 kDa by SDS-PAGE and gel-filtration, respectively. The enzyme was optimally active at 50 °C (over 30 min) and pH 5. Activity staining after PAGE showed a single band. The Km for chitin was 3 g l–1. Cu2+ and Na+ at 5 mM inhibited chitinase activity to 25% while Ca2+, Mg2+ and Ba2+ had no effect at the same concentration. The purified enzyme degraded mycelia of Aspergillus niger. |
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Keywords: | Alcaligenes xylosoxydans Aspergillus niger chitinase purification |
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