Two novel molecular isoforms of band 4.2 in Japanese Sika deer (Cervus nippon yesoensis, Heude) erythrocytes |
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Authors: | M Inaba Y Amano Y Maede |
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Affiliation: | Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. |
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Abstract: | Two molecular isoforms of band 4.2 were identified in erythrocyte membranes from 25 Japanese Sika deer (Ceryus nippon yesoensis, Heude) based on specific immunorecognition with anti-human band 4.2. These two variants, designated 4.2/78 and 4.2/76, had respective relative molecular weights (Mr) of 78,000 and 76,000 on sodium dodecyl sulfate-polyacrylamide electrophoresis gels and showed similar profiles after limited proteolysis, exhibiting identity in primary structure. 25 adult Sika deer could be divided into two groups according to the 4.2/78:4.2/76 ration, indicating a genetic control in the expression of the molecular isoforms of band 4.2. Both polypeptides were completely retained in cytoskeletal protein-depleted membranes and could be removed by alkaline extraction, suggesting that both proteins contribute to the association of membrane proteins. |
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