Lack of interaction in recombinant human FSH receptor and both TSAb and TSBAb |
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Authors: | Tano M Minegishi T Murakami M Ibuki Y Miyamoto K |
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Affiliation: | Department of Obstetrics and Gynecology, School of Medicine, Gunma University, Maebashi, Japan. |
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Abstract: | Since cross-reactivity of TSH with the human FSH receptor has been reported, in this study we tested the effect of thyroid-stimulating antibody (TSAb) and thyroid stimulation-blocking antibody (TSBAb) on Chinese hamster ovary cells expressing human FSH receptor (CHO-hFSH-R cells). We examined the TSBAb activity of sera from hypothyroid patients who had a positive TBII to determine whether these sera also block the effect of FSH on CHO-hFSH-R cells. Although human FSH I-3 (0.25-16 ng/ml) stimulated the production of intracellular cAMP in CHO-hFSH-R cells with dose-responsive manner, neither TSAb nor TSBAb had such an effect on the cells. |
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