Gastric type H+,K+-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential |
| |
Authors: | Shibata Toshiaki Hibino Hiroshi Doi Katsumi Suzuki Toshihiro Hisa Yasuo Kurachi Yoshihisa |
| |
Institution: | Div. of Molecular and Cellular Pharmacology, Department of Pharmacology, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan. |
| |
Abstract: | Cochlear endolymph has a highly positive potential of approximately +80 mV known as the endocochlear potential (EP). The EP is essential for hearing and is maintained by K+ circulation from perilymph to endolymph through the cochlear lateral wall. Various K+ transport apparatuses such as the Na+,K+-ATPase, the Na+-K+-2Cl cotransporter, and the K+ channels Kir4.1 and KCNQ1/KCNE1 are expressed in the lateral wall and are known to play indispensable roles in cochlear K+ circulation. The gastric type of the H+,K+-ATPase was also shown to be expressed in the cochlear lateral wall (Lecain E, Robert JC, Thomas A, and Tran Ba Huy P. Hear Res 149: 147154, 2000), but its functional role has not been well studied. In this study we examined the precise localization of H+,K+-ATPase in the cochlea and its involvement in formation of EP. RT-PCR analysis showed that the cochlea expressed mRNAs of gastric 1-, but not colonic 2-, and -subunits of H+,K+-ATPase. Immunolabeling of an antibody specific to the 1 subunit was detected in type II, IV, and V fibrocytes distributed in the spiral ligament of the lateral wall and in the spiral limbus. Strong immunoreactivity was also found in the stria vascularis. Immunoelectron microscopic examination exhibited that the H+,K+-ATPase was localized exclusively at the basolateral site of strial marginal cells. Application of Sch-28080, a specific inhibitor of gastric H+,K+-ATPase, to the spiral ligament as well as to the stria vascularis caused prominent reduction of EP. These results may imply that the H+,K+-ATPase in the cochlear lateral wall is crucial for K+ circulation and thus plays a critical role in generation of EP. hydrogen, potassium-adenosine triphosphatase; stria vascularis; spiral ligament |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
| 点击此处可从《American journal of physiology》浏览原始摘要信息 |
| 点击此处可从《American journal of physiology》下载免费的PDF全文 |
|