Apple tissue culture contamination by <Emphasis Type="Italic">Rhodotorula</Emphasis> spp.: Identification and prevention |
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Authors: | Email author" target="_blank">J?Kolozsvári NagyEmail author S?Sule J?P?Sampaio |
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Institution: | (1) Plant Protection Institute of the Hungarian Academy of Sciences, P.O. Box 102, 1525 Budapest, Hungary;(2) CREM, SABT, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Lisbon, Portugal |
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Abstract: | Summary Shool cultures of apple cv. Pinova were contaminated with faint pink pigmented yeast. Yeast isolates were identified as Rhodotorula slooffiae with standard physiological methods and molecular analysis. Growth of isolated yeasts was tested against different fungicides.
The following fungicides inhibited the growth of yeast isolates, and were not phytotoxic to apple shoots at concentrations
lower than the minimal phytotoxic concentrations (MPC): ProClin? 300, mancozeb, triforine, myclobutanil, thiabendazole, mancozeb+zoxamid, and silver nitrate. Some fungicides inhibited growth
of yeasts, but were phytotoxic. These included miconazole, PPM™, copper sulfate, potassium sorbate, and cycloheximide. Benomyl was not phytotoxic, but was effective only at high doses.
Decontamination of shoots was achieved using a combination of two treatments. Shoots were first soaked in half-strength Murashige
and Skoog (MS) liquid medium containing silver nitrate (588μM) and Silvet 77 (0.01%) for 1–2h, and then transferred to a solidified MS medium containing both mancozeb (15mgl−1) and thiabendazole (40mgl−1). |
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Keywords: | isothiazolones mancozeb micropropagation silver nitrate thiabendazole yeast |
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