|
|||||
|
|
Iterative Combinatorial Mutagenesis as an Effective Strategy for Generation of Deacetoxycephalosporin C Synthase with Improved Activity toward Penicillin G |
|
| Authors: | Junjie Ji Keqiang Fan Xiuyun Tian Xia Zhang Yuxiu Zhang Keqian Yang |
| Affiliation: | aSchool of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing, People''s Republic of China;bState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, People''s Republic of China |
| Abstract: | An iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase of Streptomyces clavuligerus (scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM. |
| Keywords: | |