Detection of a Bocavirus Circular Genome in Fecal Specimens from Children with Acute Diarrhea in Beijing,China |
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| Authors: | Hui Zhao Linqing Zhao Yu Sun Yuan Qian Liying Liu Liping Jia You Zhang Huijin Dong |
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| Affiliation: | 1. Graduate School of Peking Union Medical College, Beijing, China.; 2. Laboratory of Virology, Capital Institute of Pediatrics, Beijing, China.; Columbia University, United States of America, |
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| Abstract: | To determine if human bocavirus 2 (HBoV2) has a circular genome similar to the head-to-tail sequence of HBoV1 and the episomal form of HBoV3, 15 HBoV2 positive samples identified from 553 stool specimens from children with acute diarrhea were tested for a head-to-tail sequence using TaqMan-based real-time PCR. A circular genome with a head-to-tail sequence was identified in one (BJQ435) out of 15 samples tested by nested PCR. The complete circular genome of HBoV2-C1 (BJQ435) was 5307 nt in length and was flanked with a 520 nt-long terminal non-coding region (NCR). The secondary structure of HBoV2 -C1 had some differences compared to HBoV3-E1 ({"type":"entrez-nucleotide","attrs":{"text":"JN086998","term_id":"340746301","term_text":"JN086998"}}JN086998). Our study indicates that the HBoV genome exists in the form of a head-to-tail monomer and provides more information for understanding the HBoV replication mechanism. |
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