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Diamine oxidase from millet catalyzes the oxidation of 1, 3-diaminopropane
Authors:Howlader M A Awal  Eiji Hirasawa
Institution:(1) Department of Biology, Faculty of Science, Osaka City University, 558 Osaka, Japan
Abstract:Diamine oxidase was purified sixty-fold from millet shoots. The partially purified enzyme of 150 kDa oxidized 1, 3-diaminopropane (1, 3-DAP) to 3-aminopropionaldehyde. The Km values were 9.1×10−5M for 1, 3-DAP and 6.3×10−4M for putrescine. Extracts of shoots of prosomillet, maize and barley also contained an activity that oxidized 1, 3-DAP.
Keywords:3-Aminopropionaldehyde  Diamine oxidase  1  3-Diaminopropane            Setaria italica
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