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Enhanced efficiency of a capillary-based biosensor over an optical fiber biosensor for detecting calpastatin |
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| Authors: | Bratcher C L Grant S A Vassalli J T Lorenzen C L |
| Institution: | aDepartment of Animal Sciences, University of Missouri, Columbia, MO 65211, USA bDepartment of Biological Engineering, University of Missouri, Columbia, MO 65211, USA cDepartment of Food Science, University of Missouri, Columbia, MO 65211, USA |
| Abstract: | A capillary-based optical biosensor has been developed to detect calpastatin, an indicator of meat tenderness. Longissimus muscle samples (n = 11) were extracted from beef carcasses at 0 and 48 h post-mortem. These samples were assayed for calpastatin by traditional laboratory methods and with a newly developed capillary tube biosensor as well as for Warner–Bratzler shear force (WBSF) and crude protein and the responses were compared. Additionally, the response from the capillary-based biosensor was compared to a previously developed optical fiber biosensor. When the 0 and 48 h sampling periods were combined, the capillary tube biosensor was moderately accurate in predicting calpastatin activity (R2 = 0.6058). There was less variation in the 0 h capillary tube biosensor compared to the 0 h pre-column (P = 0.006) and post-column optical fiber biosensors (P = 0.047), therefore the capillary tube biosensor is a more precise system of measurement. This research further advances the development of a calpastatin biosensor and makes online assessment one step closer to reality. |
| Keywords: | Biosensor Calpastatin Fluorescence Beef Tenderness |
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