| 渗透压调控对裂殖壶菌发酵产DHA的影响 |
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| 引用本文： | 曾艳,冯云,仝倩倩,任路静,黄和. 渗透压调控对裂殖壶菌发酵产DHA的影响[J]. 生物加工过程, 2012, 0(6): 24-28 |
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| 作者姓名： | 曾艳 冯云 仝倩倩 任路静 黄和 |
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| 作者单位： | [1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009 [2]淮南师范学院,淮南232001 |
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| 基金项目： | 教育部新世纪优秀人才支持计划资助项目（NCET-09-0157）;霍英东教育基金资助项目（123014）;江苏省高校产业化推进项目（JH09-30）;江苏省六大高峰人才项目（2008-D-63） |
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| 摘 要： | 考察了不同渗透胁迫(0、10、20、30和40 g/L NaCl)对裂殖壶菌HX-308发酵产DHA及脂肪酸构成的影响。结果表明:20 g/L NaCl最有利于裂殖壶菌生长和DHA积累,生物量、总脂肪酸含量、DHA产量及DHA占生物量的比值分别为73 g/L、10.7 g/L、5.0 g/L和68 mg/g,并且DHA在总脂肪酸中所占百分比最高,为45.2%。此外,在低渗透压(10 g/L NaCl)条件下,添加40 mmol/L甘氨酸甜菜碱,DHA产量与未添加相比提高了28.21%;在高渗透压(40 g/L NaCl)条件下添加40 mmol/L海藻糖,DHA产量提高了46.84%;表明添加适量的外源相容性溶质能有效地促进裂殖壶菌积累DHA。
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| 关 键 词： | 裂殖壶菌 DHA 渗透压 相容性溶质 |
Fermentation regulation of DHA production by Schizochytrium sp. based on osmotic pressure |
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| ZENG Yan,FENG Yun,TONG Qianqian,REN Lujing,HUANG He. Fermentation regulation of DHA production by Schizochytrium sp. based on osmotic pressure[J]. Chinese Journal of Bioprocess Engineering, 2012, 0(6): 24-28 |
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| Authors: | ZENG Yan FENG Yun TONG Qianqian REN Lujing HUANG He |
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| Affiliation: | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China ; 2. Huainan Normal University, Huainan 232001, China) |
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| Abstract: | The effects of different osmotic pressures （0,10,20,30 and 40 g/L NaC1） on DHA accumula- tion and fatty acids composition of Schizochytrium sp. HX-308 were studied. 20 g/L salinity was proved to be beneficial for cell growth and DHA production. Cell dry weight （DCW） , total content of fatty acids,DHA yield, and DHA concentration on DCW were the highest, around 73 g/L, 10.7 g/L, 5.0 g/L, and 68 mg/g, respectively. The DHA percentage of total fatty acids also reached the maxi- mum of 45.2 %. In addition, under the conditions of 40 mmol/L glyeine betaine addition under low sa- linity （ 10 g/L NaCl） and 40 mmol/L trehalose addition under high salinity （40 g/L NaC1） DHA yield could increase by 28.21% and 46. 84% , respectively, thus demonstrating the positive effect of compatible solute addition to be effective for DHA production by Schizochytrium sp. |
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| Keywords: | Schizochytrium sp. docosahexaenoic acid osmotic pressure compatible solutes |
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