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Evaluation of radioactive and nonradioactive gene probes and cell culture for detection of poliovirus in water samples.
Authors:N J Moore and A B Margolin
Abstract:Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.
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