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Chronic systemic treatment with epidermal growth factor in the rat increases the mucosal surface of the small intestine |
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| Authors: | Lars Vinter-Jensen Morten Smerup Peter Kissmeyer-Nielsen Steen Seier Poulsen |
| Affiliation: | a Department of Clinical Biochemistry, Aarhus University Hospital, Nørrebrograde 44, DK-8000, Aarhus C, Denmark b Department of Surgery L, Amtssygehuset, Aarhus University Hospital, Aarhus, Denmark c Stereological Research Laboratorium, University of Aarhus, Aarhus, Denmark d Institute of Medical Anatomy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark |
| Abstract: | We examined the effects of treatment with human recombinant epidermal growth factor (EGF) on the functioning small intestine in the rat. Male Wistar rats, 7–8 weeks old, were treated with EGF administered subcutaneously in doses of 0 (n = 7) or 150 μg/kg/day (n = 8) for 4 weeks. The histological composition and mucosal surface area of the perfusion-fixed small intestine was quantified with stereological principles. The length of the gut remained unchanged. The amount of tissue and surface area per length of gut (median (ranges)) were increased from 117 (101–131) mg/cm and 2.6 (2.1–3.5) cm2/cm in the controls to 146 (138–152) mg/cm and 3.5 (2.5–3.8) cm2/cm for the complete small intestine (both comparisons p < 0.02). The weight increase was due to mucosal growth in all parts of the intestine, whereas the surface area was only increased in proximal and middle parts. It is concluded that EGF treatment in rats increases the mucosal weight and surface area of the functioning small intestine. |
| Keywords: | Growth factor Intestine Stereology Surface area |
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