Computer-assisted motion analysis of sperm from the common carp |
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| Authors: | G P Toth S A Christ H W McCarthy † J A Torsella ‡ M K Smith |
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| Institution: | Cellular and Biochemical Markers Branch, Ecological Monitoring Research Division, Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH 45268;Technology Applications, Inc. Cincinnati, OH;Oak Ridge Institute for Science and Education, U.S. Department of Energy, Oak Ridge, TN, U.S.A. |
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| Abstract: | Computer-assisted semen analysis (CASA) technology was applied to the measurement of sperm motility parameters in the common carp Cyprinus carpio. Activated sperm were videotaped at 200 frames s−1 and analysed with the CellTrak/S CASA research system. The percentage of motile cells and both sperm head curvilinear velocity and straight-line velocity were measured following exposure of carp sperm to three predilution conditions and activation in media of differing ionic strengths and osmotic pressures. The highest percentage of motile sperm was obtained following predilution of sperm in seminal plasma and activation in Na-HEPES buffer pH 8.0. This level of motility was equalled after predilution in 200 m m KCl for 2 h. Straight-line velocities and curvilinear velocities of 130 μm s−1 and 210 μm s−1, respectively, were observed. Duration of motility was higher under seminal plasma predilution conditions (over 50% motile sperm at 55 s post-activation). The application provides a sound basis for the assessment of Sperm Characteristics in fish. |
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| Keywords: | carp sperm motility computer-assisted semen analysis videomicroscopy |
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