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Regulation of acetate and acetyl-CoA converting enzymes during growth on acetate and/or glucose in the halophilic archaeon Haloarcula marismortui |
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| Authors: | Bräsen Christopher Schönheit Peter |
| Institution: | 1. Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav), Departamento de Biotecnología y Bioingeniería, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico;2. Universidad Francisco de Paula Santander, Av. Gran Colombia 12E-96, San José de Cúcuta, Colombia;1. School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150009, PR China;2. School of Management, Harbin Institute of Technology, 92 West Dazhi Street, Nan Gang District, Harbin 150001, PR China;3. Department of Geography, University of North Carolina, 237 Graham Building, 1009 Spring Garden St, Greensboro, NC 27412, USA;1. Hawaii Institute of Geophysics and Planetology, University of Hawaii, Honolulu, HI 96822, USA;2. Department of Geosciences, Stony Brook University, Stony Brook, NY 11794, USA;1. SRM Research Institute, SRM University, Kattankulathur, 603203, India;2. Department of Physics and Nanotechnology, SRM University, Kattankulathur, 603203, India |
| Abstract: | Haloarcula marismortui formed acetate during aerobic growth on glucose and utilized acetate as growth substrate. On glucose/acetate mixtures diauxic growth was observed with glucose as the preferred substrate. Regulation of enzyme activities, related to glucose and acetate metabolism was analyzed. It was found that both glucose dehydrogenase (GDH) and ADP-forming acetyl-CoA synthetase (ACD) were upregulated during periods of glucose consumption and acetate formation, whereas both AMP-forming acetyl-CoA synthetase (ACS) and malate synthase (MS) were downregulated. Conversely, upregulation of ACS and MS and downregulation of ACD and GDH were observed during periods of acetate consumption. MS was also upregulated during growth on peptides in the absence of acetate. From the data we conclude that a glucose-inducible ACD catalyzes acetate formation whereas acetate activation is catalyzed by an acetate-inducible ACS; both ACS and MS are apparently induced by acetate and repressed by glucose. |
| Keywords: | Haloarcula marismortui Acetate formation Acetate activation ADP-forming acety-CoA synthetase AMP-forming acetyl-CoA synthetase Overflow metabolism Catabolite repression |
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