Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars |
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Authors: | B S Dien N N Nichols R J Bothast |
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Institution: | (1) Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA, US |
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Abstract: | Recombinant Escherichia coli have been constructed for the conversion of glucose as well as pentose sugars into L-lactic acid. The strains carry the lactate dehydrogenase gene from Streptococcus bovis on a low copy number plasmid for production of L-lactate. Three E. coli strains were transformed with the plasmid for producing L-lactic acid. Strains FBR9 and FBR11 were serially transferred 10 times in anaerobic cultures in sugar-limited medium containing
glucose or xylose without selective antibiotic. An average of 96% of both FBR9 and FBR11 cells maintained pVALDH1 in anaerobic
cultures. The fermentation performances of FBR9, FBR10, and FBR11 were compared in pH-controlled batch fermentations with
medium containing 10% w/v glucose. Fermentation results were superior for FBR11, an E. coli B strain, compared to those observed for FBR9 or FBR10. FBR11 exhausted the glucose within 30 h, and the maximum lactic acid
concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic
acid (0.5 g/l). Journal of Industrial Microbiology & Biotechnology (2001) 27, 259–264.
Received 05 November 2000/ Accepted in revised form 03 July 2001 |
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Keywords: | : lactic acid production Escherichia coli fermentation pentoses |
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