Characterization of a Whole Set of tRNA Molecules in an Aerobic Hyper-thermophilic Crenarchaeon, Aeropyrum pernix K1 |
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Authors: | Yamazaki, Syuji Kikuchi, Hisashi Kawarabayasi, Yutaka |
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Affiliation: | 1National Institute of Technology and Evaluation 2-49-10 Nishihara, Shibuya, Tokyo 151-0066, Japan 2National Institute of Advanced Industrial Science and Technology (AIST) Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan |
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Abstract: | The tRNA molecule has an important role in translation, thefunction of which is to carry amino acids to the ribosomes.It is known that tRNA is transcribed from tRNA genes, some ofwhich, in Eukarya and Archaea, contain introns. A computationalanalysis of the complete genome of Aeropyrum pernix K1 predictedthe presence of 14 intron-containing tRNA genes. To elucidatewhether these introns are actually processed in living cellsand what mechanism detects the intron regions, cDNAs for prematureand mature forms of the tRNA molecules transcribed from theintron-containing tRNA genes in the model aerobic acidothermophiliccrenarchaeon, A. pernix K1 were identified and analyzed. A comparisonbetween the nucleotide sequences of these two types of cDNAsindicated that the intron regions of the tRNA molecules wereindeed processed in A. pernix K1 living cells. Some cDNA clonesshowed that the actual splicing positions were different fromthose predicted by computational analysis. However, the bulgehelixbulgestructure, which has been previously identified in exonintronboundaries of archaeal tRNA genes, was evident in all boundaryregions confirmed in this work. These results indicate thatthe generally described mechanism for tRNA processing in Archaeais utilized for processing the intron region of the tRNA moleculesin A. pernix K1. |
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Keywords: | tRNA intron splicing bulge helix bulge structure genome Crenarchaeon Aeropyrum pernix K1 |
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