Oxidation of reduced cytochrome c by hydrogen peroxide. Implications for superoxide assays |
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Authors: | P L Vandewalle N O Petersen |
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Affiliation: | Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada |
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Abstract: | Hydrogen peroxide, formed directly or as a product of superoxide dismutation, can oxidize ferrocytochrome c at rates comparable to those at which ferricytochrome c is reduced by superoxide. This reoxidation can significantly affect estimates of rates and amounts of superoxide production using absorbance changes for cytochrome c at 550 nm as the assay. The oxidation can be inhibited by catalase. |
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