Azide-resistant mutants ofAzorhizobium caulinodans with enhanced symbiotic effectiveness |
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Authors: | I Saini S S Sindhu K R Dadarwal |
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Institution: | (1) Department of Microbiology, CCS Haryana Agricultural University, 125 004 Hisar, India |
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Abstract: | Azide-resistant mutants ofAzorhizobium caulinodans strains Sb3, S78, SrR13 and SrS8 were isolated and screened for nitrate reductase activity. Selected nitrate reductase negative
mutants were inoculated onSesbania bispinosa andS. rostrata under sterile conditions in chillum jars to study their symbiotic behavior. Azide-resistant mutants exhibited either similar
or higher symbiotic effectiveness than the parent strain after 30 d of plant growth. Nodule mass, nitrogenase activity and
uptake hydrogenase activity of the mutants varied depending on the host as well as on the plant growth stage. In comparison
to wild-type parent strains, four azide-resistant mutants, Sb3Az18, S78Az21, SrR13Az17 and SrS8Az6 showed significant increase
in nodulation and nitrogen fixation as well as shoot dry mass of the inoculated plants. |
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