Isolation of a psychrotrophic Azospirillum sp. and characterization of its extracellular protease |
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Authors: | Oh K H Seong C S Lee S W Kwon O S Park Y S |
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Affiliation: | Department of Microbiology, Inje University, South Korea. |
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Abstract: | A novel psychrotrophic bacterium secreting a protease was isolated from a mountain soil in Korea. On the basis of a 16S rDNA sequence analysis and physiological properties, the isolate was identified as an Azospirillum sp. The protease purified from the culture supernatant was a monomer in its native form with an apparent molecular mass of 48.6 kDa on SDS-PAGE. The protease was active in a broad pH range around 8.5 and at temperatures up to 40 degrees C and stable at temperatures below 30 degrees C for 3 days. The proteolytic activity was inhibited by iodoacetamide and EDTA. The Mg2+ ion did not activate the enzyme much but reversed the inhibition by EDTA, suggesting that the protease belongs to a cysteine protease stabilized by the Mg2+ ion. |
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Keywords: | Psychrotroph Azospirillum sp. 16S rRNA sequence Extracellular protease Purification |
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