The mitochondrial genome of fission yeast: inability of all introns to splice autocatalytically, and construction and characterization of an intronless genome |
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Authors: | Bernd Schäfer Ana Maria Merlos-Lange Carola Anderl Franz Welser Manfred Zimmer and Klaus Wolf |
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Institution: | (1) Institut für Mikrobiologie und Weinforschung der Johannes Gutenberg-Universität, Becherweg 15, W-6500 Mainz, Germany;(2) Europäisches Patentamt, W-8000 München, Germany;(3) Max Planck-Institut für Biochemie, Am Klopferspitz 18a, W-8033 Martinsried, Germany |
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Abstract: | Summary In this paper we report the inability of four group I introns in the gene encoding subunit I of cytochrome c oxidase (cox1) and the group II intron in the apocytochrome b gene (cob) to splice autocatalytically. Furthermore we present the characterization of the first cox1 intron in the mutator strain ana
r
-14 and the construction and characterization of strains with intronless mitochondrial genomes. We provide evidence that removal of introns at the DNA level (termed DNA splicing) is dependent on an active RNA maturase. Finally we demonstrate that the absence of introns does not abolish homologous mitochondrial recombination.Abbreviations
cox1, cox2, cox3
genes encoding subunits 1, 2 and 3 of cytochrome
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c
oxidase
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cob
gene encoding apocytochrome b
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cox1I1, cox1I2a, cox1I2b, cox1I3
introns in cox1
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cox1Ix
+/–
indicates the presence or absence of the intron either in the native gene or after intron DNA excision
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cox1Ix
is a deletion in the intron leading to respiratory deficiency |
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Keywords: | Schizosaccharomyces pombe Mitochondrial genome Intron DNA excision Autocatalytic RNA splicing Recombination |
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