A monoclonal antibody to swine erythrocytes recognizes the B blood group on the major glycophorin |
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| Authors: | A Gonzá lez,M Friend,A Moreno,C O Pintado,P Vö geli,D Llanes |
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| Affiliation: | Departamento Genética, Instituto de Agricultura Sostenible, (CSIC), Facultad de Veterinaria, Avd. Medina Azahara s/n, 1405 Córdoba, Spain;Nutztierwissenschaften, Gruppe Züchtungsbiologie, ETH-Zentrum. Swiss Federal Institute of Technology, Zurich, CH-8092 Switzerland |
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| Abstract: | Recently monoclonal antibodies (mAbs) to swine red blood cells have been described. One of them (1AC11) was specific for the major swine glycoprotein with a molecular weight of 45 kDa and another mAb, 2G2, recognized the B a allele in the B system of swine blood groups. Immunoblotting experiments to characterize the mAb 2G2 indicated that it reacts with an antigen of 45 kDa, present on the aqueous phase, glycophorin fraction, of swine red blood cells with the B a allele and does not react with B bBb homozygous cells. The antigen recognized by 2G2 has the same characteristics as the major glycophorin recognized by 1AC11, so we can conclude that the B system of the swine blood group is on the major glycophorin of swine erythrocyte membranes. |
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| Keywords: | glycophorin A monoclonal antibodies swine blood groups |
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