Polysomes are associated with microtubules in fertilized eggs of Chinese pine (Pinus tabulaeformis) |
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Authors: | Yuzhen Han Jingjuan Yu Fengli Guo Simon C Watkins |
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Institution: | (1) State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Science, China Agricultural University, Beijing;(2) Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania |
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Abstract: | Summary. Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that
polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of
various size and shape were randomly distributed in the cytoplasm of the eggs before fertilization. Single MTs or clusters
were observed to be free of polysomes at this stage. Upon fertilization, all polysomes were attached to MTs, and this association
persisted until the formation of the polarized embryo. Thereafter, the polysomes spread into the cytoplasm and no polysome-MT
association was observed in the embryo. Some of the polysomes were attached to one end of the MTs, while others appeared to
form contacts along their entire length. No polysome-microfilament association was observed at any stage of the development.
The polysome-MT association may provide a mechanism for MT-dependent mRNA localization in early embryo development of this
plant.
Correspondence and reprints: Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, U.S.A. |
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Keywords: | : Pinus tabulaeformis Microtubule mRNA localization Polysome Immunogold |
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