A hemoprotein from azotobacter containing non-heme iron: isolation and crystallization |
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Authors: | W A Bulen J R LeComte S Lough |
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Affiliation: | Charles F. Kettering Research Laboratory Yellow Springs, Ohio 45387 USA |
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Abstract: | A new type of hemoprotein was isolated from extracts of using a heat step, application of the protamine-cellulose phosphate procedure, and precipitation with MgCl2. Crystallization was achieved using low concentration of MgCl2. The protein was shown to be a unique cytochrome of the b type containing up to 20% non-heme iron but no “acid labile sulfur”. Both the heme and non-heme iron appear to be functional redox components. |
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