AGEPC (platelet activating factor) induced stimulation of rabbit platelets: effects on phosphatidylinositol, di- and triphosphoinositides and phosphatidic acid metabolism |
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Authors: | S D Shukla D J Hanahan |
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Affiliation: | Department of Biochemistry The University of Texas Helath Science Center San Antonio, Texas 78284 USA |
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Abstract: | Stimulation of washed rabbit platelets with AGEPC (1-O-alkyl-2-acetyl--glyceryl-3-phosphorylcholine) caused a 15–20% decrease in their phosphatidylinositol level within 15 seconds without affecting other major classes of phospholipids. In the same time frame the level of phosphatidic acid (PA) increased dramatically some four fold. LysoGEPC, which is inactive in stimulating rabbit platelets, did not cause any change in PI or PA. When [32Pi] was present during the stimulation of platelets by AGEPC, the incorporation of radiolabel into PI-4-phosphate (DPI), PI-4,5-bis phosphate (TPI) and PA was enhanced significantly within one minute while the incorporation into PI increased only after one minute. These results clearly established that AGEPC induced stimulation of rabbit platelets was associated with the metabolism of inositol phospholipids and phosphatidic acid. The relevance of these findings to the mode of action of AGEPC and Ca2+ mobilization is also discussed. |
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Keywords: | AGEPC DPI diphosphoinositide TPI triphosphoinositide PA phosphatidic acid PC phosphatidylcholine PE phosphatidylethanolamine PI phosphatidylinositol PS phosphatidylserine |
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