Erythrocyte D-glucose transport activity in reconstituted model membranes of different lipid composition |
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| Authors: | B Cestaro G Cervato O Carandente A M Girardi G Pozza |
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| Institution: | Dipartimento di Chimica e Biochimica Medica, Facoltà di Medicina e Chirurgia, Milano, Italy. |
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| Abstract: | The stereospecific influx of D-glucose into liposomes formed on sonication of different glyco- and phospholipids with transport proteins from human erythrocyte ghosts solubilized with Triton x-100 was measured as an index of their total D-glucose transport activity. Specific D-glucose transport increased when acidic phospho- and glycolipids (especially sulfatide) were added to the phosphatidylcholine bilayers of the model membranes while cholesterol strongly inhibited the process. The modulation of D-glucose transport activity and its possible correlation with the lipid composition and the chemico-physical state of the erythrocytes is discussed. |
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