Effectiveness of bulking procedures in measuring population-pairwise similarity with dominant and co-dominant genetic markers |
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| Authors: | Y B Fu |
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| Institution: | (1) Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7 N 0X2, Canada e-mail: fuy@em.agr.ca Tel.: +1-306–956–7642, Fax: +1-306–956–7246, CA |
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| Abstract: | Two bulking procedures (bulking individuals before and after genotyping) are commonly applied in similarity based studies
of genetic distance at the population or higher level, but their effectiveness is largely unknown. In this study, expected
population-pairwise similarity for both bulking procedures is derived with dominant and co-dominant diallelic markers. Numerical
examples for the derived formulae are given with up to ten individuals randomly selected from each population. The procedure
of bulking individuals after genotyping with either marker system is generally more informative than the procedure of bulking
individuals before genotyping, because the former incorporates the information from marker alleles of intermediate frequency.
Both procedures are effective with 5–10 individuals selected randomly from either population, but the procedure of bulking
before genotyping requires a genotyping effort several-fold less than the procedure of bulking after genotyping. For either
bulking procedure, a co-dominant marker system is generally more informative than a dominant marker system.
Received: 20 October 1999 / Accepted: 11 November 1999 |
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| Keywords: | Population similarity Genetic relationship Co-dominant marker Dominant marker Bulk analysis |
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