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Two-dimensional electrophoresis of 1D-encoded B and D glutenin subunits in common wheats with similar omega gliadins
Authors:S. Masci  E. Porceddu  D. Lafiandra
Affiliation:(1) Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis, 01100 Viterbo, Italy
Abstract:Gli-D1-encoded omega gliadins of bread wheats show little variation; their electrophoretic patterns can be classified into two main groups which broadly resemble the patterns found in the cultivars Chinese Spring and in Cheyenne. B and D subunits of low molecular weight glutenin encoded by the chromosome 1D lociGlu-D3 andGli-D1, respectively, also showed little variation. D subunits were found only in bread wheats with ldquoChinese Spring-typerdquo omega gliadins and they all exhibited the same electrophoretic pattern. This material also showed very similar B subunits. ldquoCheyenne-typerdquo bread wheats displayed the same electrophoretic distribution of chromosome 1D-encoded B subunits, although they were slightly different from that found in Cheyenne itself.This work was supported by the Eclair Programme of the Commission of Europe and the Community.
Keywords:low molecular weight glutenin subunits  omega gliadins  wheat storage protein  D genome  breadmaking quality
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