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Staining Senile Plaques using Bodian's Method Modified with Methenamine |
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| Authors: | Hirorni Kondoh Masaaki Matsushita Kenji Kosaka Noriyuki Miyazaki |
| Institution: | a Department of Neuropathology, Tokyo Institute of Psychiatry, Tokyo, Japan b Department of Psychiatry, Faculty of Medicine, University of Tokyo, Tokyo, Japan c Department of Psychiatry, Yokohama City University School of Medicine, Yokohama, Japan d Department of Radioisotope, Tokyo Institute of Psychiatry, Tokyo, Japan |
| Abstract: | A new method is presented for staining various types of senile plaques isolated from the brains of patients with Alzheimer type dementia and related diseases in paraffin embedded sections using a modified Bodian's method with methenamine. This methenamine-Bodian method made it possible to observe diffuse plaques and other amyloid deposits which are barely detected by Bodian's original method. The staining of senile plaques by the method presented here was comparable to that of immunostaining with anti-β-protein. The new method also stained neurofibrillary tangles. Therefore, the methenamine-Bodian method could be widely used for the detection of senile changes in paraffin embedded sections from autopsied human brains. |
| Keywords: | methenamine-Bodian method Bodian's method senile plaque Alzheimer type dementia |
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