The effect of light and 2,4-D on anthocyanin production in Oxalis reclinata callus |
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Authors: | N.P. Makunga J. van Staden W.A. Cress |
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Affiliation: | (1) Natal University Research Unit of Plant Growth and Development, University of Natal, Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa |
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Abstract: | In vitro cultures of O. reclinata accumulate red anthocyanin pigments. Two callus lines were established from O. reclinata, one red and the other non-pigmented. The red callus accumulated cyanidin-3-glucoside as a major pigment. Light irradiation induced anthocyanin synthesis in white callus, resulting in a heterogenous red callus line being formed. The incubation of red and white callus cultures in the dark or at low-light resulted in the repression of red pigment accumulation. The application of 2,4-D (1.0 mg l-1) inhibited pigment production in the white callus and decreased anthocyanin accumulation in the red callus. The polypeptide composition of the red and white callus lines from O. reclinata were compared using two-dimensional electrophoresis. The red callus had a larger subset of neutral and acidic polypeptides. |
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Keywords: | anthocyanins light Oxalis reclinata pigmented callus 2,4-D suspension cultures two-dimensional electrophoresis |
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