Expression Profiling and Validation of Potential Reference Genes During <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> Embryogenesis |
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Authors: | Qiwang Zhong Quanqi Zhang Zhigang Wang Jie Qi Yanjie Chen Shuo Li Yeying Sun Chunmei Li Xun Lan |
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Institution: | (1) Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, People’s Republic of China |
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Abstract: | Differential expression of genes is crucial to embryogenesis. The analysis of gene expression requires appropriate references
that should be minimally regulated during the embryonic development. To select the most stable genes for gene normalization,
the expression profiles of eight commonly used reference genes (ACTB, GAPDH, rpL17, α-Tub, EF1-α, UbcE, B2M, and 18S rRNA)
were examined during Japanese flounder (Paralichthys olivaceus) embryonic development using quantitative real-time polymerase chain reaction. It was found that all seven mRNA genes appeared
to be developmentally regulated and exhibited significant variation of expression. However, further analyses revealed the
stage-specific expression stability. Hence when normalization using these mRNA genes, the differential and stage-related expression
should be considered. 18S rRNA gene, on the other hand, showed the most stable expression and could be recommended as a suitable
reference gene during all embryonic developmental stages in P. olivaceus. In summary, our results provided not only the appropriate reference gene for embryonic development research in P. olivaceus, but also possible guidance to reference gene selection for embryonic gene expression analyses in other fish species. |
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Keywords: | Expression profiles Paralichthys olivaceus Quantitative real-time PCR Reference genes |
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