Lignin peroxidase isoforms from Streptomyces viridosporus T7A: are they a monomer based structure? |
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| Authors: | Elba PS Bon Hilton J Nascimento Jacyara MB Macedo José G Silva Jr |
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| Institution: | (1) Instituto de Química, Depto. de Bioquímica, Universidade Federal do Rio de Janeiro, CT, Bloco A, Ilha do Fundão, Rio de Janeiro, RJ, CEP 21949-900, Brasil;(2) Depto. de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, RJ, CEP 20551-013, Brasil |
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| Abstract: | Five fractions with lignin peroxidase activity were isolated by FPLC-Mono Q from a Streptomyces viridosporus culture. F4 and F5 showed the highest specific activity and degree of protein homogeneity by chromatofocusing, IEF- and gradient-PAGE. The individual analysis of F4 and F5 by FPLC-Superdex 75, showed MW that were multiples to each other (68,000; 23,000; 12,000), although by SDS PAGE a sole MW of 13,500 was obtained, indicating a monomer based structure. The amino-acid composition of F5 showed absence of sulfur amino acids. |
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| Keywords: | isoforms characterization lignin peroxidase molecular weight polymeric structure Streptomyces viridosporus |
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