(1) Department of Medicine, University of Sydney, Rm 371, Blackburn Bld D06, Western Ave, 2006 Sydney, NSW, Australia;(2) Sydney Cancer Centre, Royal Prince Alfred Hospital, 2050 Sydney, NSW, Australia
Abstract:
Transgenic research often suffers from the lack of strong tissue specific promoters and the lack of suitable antibodies for transgene detection. We have now constructed a novel prostate specific 2-in-1 Tetracycline-off (Tet-off), bicistronic expression vector, designated PbTetOIE, for transgenic research. The vector allows potent induction as well as inducible suppression of transgene expression in the prostate epithelial cells, and also allows the detection of transgene expression at the cellular level through the detection of the internal enhanced green fluorescent protein (EGFP) downstream of the transgene.