Analysis of post-implantation mouse embryos after maternal heat stress during meiotic maturation |
| |
Authors: | A P Baumgartner C L Chrisman |
| |
Institution: | Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907. |
| |
Abstract: | The effects of heat stress during oocyte maturation were studied in post-implantation mouse embryos. Virgin ICR mice were exposed to 35 +/- 1 degree C and 65 +/- 3% RH for 12.5 h beginning immediately after synchronization of ovulation with PMSG and hCG. Embryos of heat-stressed dams were developmentally heterogeneous and showed significant delays in development with as much as 48 h delayed development. Nearly 6% of these embryos were triploid, and another 2% were hyper-diploid. Development of triploid embryos was delayed more than 24 h. Nine embryos with severe developmental delay had heterogeneous chromosome constitutions. Embryo mortality before and after implantation was higher in heat-stressed dams than in controls. |
| |
Keywords: | |
|
|