Changes in cytosolic free Ca2+ in isolated parietal cells: Differential effects of secretagogues |
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| Authors: | Shmuel Muallem George Sachs |
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| Institution: | 1. Center for Ulcer Research and Education, Department of Medicine, University of California, Los Angeles, U.S.A.;2. VA Wadsworth Hospital Center, Los Angeles, CA 90073, U.S.A. |
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| Abstract: | The relationship of free cytosolic Ca2+ to secretagogue-dependent activation of acid secretion by the mammalian parietal cell was studied using quin 2 as an intracellular Ca2+ probe. The resting Ca2+]in of isolated dog parietal cells was found to be 134±11 nM. Carbachol produced a steady-state increase of Ca2+]in and its effect was blocked by atropine and Ca2+-channel blocking agents. Gastrin transiently elevated Ca2+]in and this was not affected by Ca2+-channel blocking agents. Neither histamine nor dbcAMP changed resting Ca2+]in in rabbit parietal cells. |
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| Keywords: | Secretagogue (Parietal cell) |
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