Phosphorylation of glycogen synthase in isolated rabbit hepatocytes |
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Authors: | Carlos Ciudad Anna DePaoli-Roach Peter J. Roach |
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Affiliation: | Department of Biochemistry, Indiana University School of Medicine, Indianapolis, IN 46223 U.S.A. |
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Abstract: | Specific antibodies were used to purify glycogen synthase from isolated rabbit hepatocytes that had been incubated in a medium containing [32P]phosphate. The enzyme gave rise to two main 32P-labeled CNBr fragments of electrophoretic mobilities similar to those obtained after phosphorylation of the enzyme by individual protein kinases in vitro. |
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Keywords: | Glycogen synthase Phosphorylation (Rabbit hepatocyte) |
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