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Phosphorylation of glycogen synthase in isolated rabbit hepatocytes
Authors:Carlos Ciudad  Anna DePaoli-Roach  Peter J. Roach
Affiliation:Department of Biochemistry, Indiana University School of Medicine, Indianapolis, IN 46223 U.S.A.
Abstract:Specific antibodies were used to purify glycogen synthase from isolated rabbit hepatocytes that had been incubated in a medium containing [32P]phosphate. The enzyme gave rise to two main 32P-labeled CNBr fragments of electrophoretic mobilities similar to those obtained after phosphorylation of the enzyme by individual protein kinases in vitro.
Keywords:Glycogen synthase  Phosphorylation  (Rabbit hepatocyte)
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