Transfer RNA changes in rat granulation tissue possibly related to collagen synthesis

Transfer RNAs for glycine, proline, lysine, serine and leucine were compared in developing rat granulation tissue 6 and 15 days after sterile subcutaneous implantation of pieces of cellulose sponge. The acceptance of glycine, proline and lysine by unfractionated tRNAs were ca. 30 per cent greater in tRNA derived from 15-day granulation tissue, whereas those of serine and leucine were unaltered. Cochromatography on benzoylated DEAE-cellulose of the ³H- and ¹⁴C-labeled aminoacyl-tRNAs from the two sources revealed a significant increase in the relative amount of one of the three glycyl-tRNA fractions in the 15-day granulation tissue, whereas the elution profiles for prolyl-, lysyl-, seryl-, and leucyl-tRNAs were unaltered. The changes observed suggest a causal relation to the enhanced synthesis of collagen in the late-stage granulation tissue.