Studies on electron transport associated with Photosystem I. I. Functional site of plastocyanin: Inhibitory effects of HgCl2 on electron transport and plastocyanin in chloroplasts |
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| Authors: | Mamiko KimimuraSakae Katoh |
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| Institution: | Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Hongo, Tokyo Japan |
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| Abstract: | 1. Incubation of chloroplasts with HgCl2 at a molar ratio of HgCl2 to chlorophyll of about unity, induced a complete inhibition of the methyl viologen Hill reaction, as well as methyl viologen photoreduction with reduced 2,6-dichlorophenolindophenol (DCIP) as electron donor. Photooxidation of cytochrome ? was similarly sensitive towards HgCl2, whereas photooxidation of P700 was resistant to the poison. Photoreduction of cytochrome ? and light-induced increase in fluorescence yield were enhanced by the HgCl2 treatment of chloroplasts. |
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| Keywords: | DCIP 2 6-dichlorophenolindophenol DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea PMS phenazine methosulfate |
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